Biomembranes Part Q: ATP-Driven Pumps and Related Transport: by Sidney Fleischer, Becca Fleischer PDF

By Sidney Fleischer, Becca Fleischer

The delivery volumes of the Biomembranes sequence have been initiated with Volumes a hundred twenty five and 126 of tools in Enzymology. those volumes coated delivery in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 hide ATP-Driven Pumps and similar Transport.
The subject of organic membrane shipping is a truly well timed one simply because a powerful conceptual foundation for its knowing now exists

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Additional info for Biomembranes Part Q: ATP-Driven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps

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6 and 3 rain. 0 is lower than at higher pCa values, probably because of the operation of the Ca2÷-induced Ca 2+ release mechanism. Results for three slow fibers are combined. [Reproduced from K. Horiuti, J. Physiol. ] Areas under the contracture curve (tension-time integrals) but not peak tensions are recommended as the index of the magnitude of contracture and hence of the amount of Ca 2÷ released. With an increase in the amount of Ca 2+ released peak tension does reach saturation at some point, but the tension-time integral does not because the duration of the contracture can still increase.

Physiol. 50, 255 (1966). 18 B. A. Curtis and R. S. Eisenberg, J. Gen. Physiol. 85, 383 (1982). 19 L I E . Ford and R. J. Podolsky, J. Physiol. (London) 223, 1 (1972). 20 E. W. Stephenson, J. Gen. Physiol. 71, 411 (1978). 21 j. R. Blinks, W. G. Wiet, P. Hess, and F. G. Prendergast, Prog. Biophys. Mol. Biol. 40, 1 (1982). [9_] C a 2+ RELEASE IN SKINNED SKELETAL MUSCLE FIBERS 17 Cae+ Measurement by Utilizing Contraction of the Skinned Fiber Under appropriate conditions, application of a high concentration of caffeine causes contraction of skinned fibers in a relaxing solution due to the release of Ca 2÷ from the SR.

Skinning can be carried out either mechanically or chemically. Mechanical Skinning Mechanical skinning can be performed in either an oiP ° or a relaxing solution. 6 The entire surface membrane can be removed ~°or the fibers can be split into two (or more) longitudinal pieces to get partially skinned fibers. 6 The main difference between completely skinned fibers and partially skinned fibers is that, whereas in completely skinned fibers the disrupted T system membrane is likely to be sealed off and a potential difference across the T membrane may be reestablished with the aid of active sodium transport, in partially skinned fibers (if appropriately prepared) the mouths of the T tubules on the remaining sarcolemma may still be open to the outside and no ionic gradient across the T membrane can be established.

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