Download e-book for iPad: Avidin-Biotin Technology by John N. Abelson, Melvin I. Simon, Meir Wilchek, Edward A.

By John N. Abelson, Melvin I. Simon, Meir Wilchek, Edward A. Bayer

The avidin-biotin complicated has been used for isolation (affinity chromatography), localization (affinity cytochemistry, telephone cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). lately, utilization of the approach has been prolonged to incorporate different parts. This quantity covers those new functions and methodologies together with hybridoma know-how, bioaffinity sensors, affinity focusing on, and drug supply, in addition to cross-linking, immobilization, and fusogenic experiences

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Download PDF by John N. Abelson, Melvin I. Simon, Meir Wilchek, Edward A.: Avidin-Biotin Technology

The avidin-biotin advanced has been used for isolation (affinity chromatography), localization (affinity cytochemistry, phone cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). lately, utilization of the method has been prolonged to incorporate different components. This quantity covers those new functions and methodologies together with hybridoma know-how, bioaffinity sensors, affinity concentrating on, and drug supply, in addition to cross-linking, immobilization, and fusogenic experiences

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H. Gilad, J. Histochem. Cytochem. 29, 687 (1981). 2t5 M. Berger, T. A. Gaither, R. M. Cole, T. M. Chused, C. H. Hammer, and M. M. Frank, Mol. Immunol. 19, 857 (1982). 216 D. Safer, J. Hainfeld, J. S. Wall, and J. E. Reardon, Science 218, 290 (1982). 217 j. A. Ripellino, M. M. Klinger, R. U. Margolis, and R. K. Margolis, J. Histochem. Cytochem. 33, 1060 (1985). 218 K. Sutoh, K. Yamamoto, and T. Wakabayashi, Proc. Natl. Acad. Sci. A. 83, 212 (1986). 2~9 R. J. Seitz, K. Heininger, G. Schwendemann, and K.

Methods 73, 83 (1984). ~9S. A. Fuller, A. Philips, and M. S. Coleman, Biochem. J. 231, 105 (1985). 20 W. T. Lee and D. H. Conrad, J. Exp. Med. 159, 1790 (1984). 2~ T. Nakajima and G. Delespesse, Eur. J. lmmunol. 16, 809 (1986). 22 G. Redeuilh, C. -E. Baulieu, J. Biol. Chem. 260, 3996 (1985). 23 B. Manz, A. Heubner, I. -J. Grill, and K. Pollow, Eur. J. Biochem. 131, 333 (1983). 24 E. Hazum, I. Schvartz, Y. Waksman, and D. Keinan, J. Biol. Chem. 261, 13043 (1986). 25 F. M. Finn, G. Titus, D. Horstman, and K.

Gawinowicz Kolks, C. E. Argarana, and C. R. Cantor, J. Biol. Chem. 262, 13933 (1987). s3 p. C. Weber, D. H. Ohlendorf, J. J. Wendoloski, and F. R. Salemme, Science 243, 85 (1989). 54 See M. Wilchek and E. A. Bayer, this volume [3]. TABLE II EARLIER COVERAGE OF AVIDIN--BIOTIN TECHNIQUES IN THIS SERIES Subject Biotin Assays for biotin Biotin analogs Biotin derivatives Biotinylated proteins Biotinylated hormones Biotinylated glycoconjugates Biotinyl lipids Biotinylated nucleic acids Immobilized biotin Iminobiotin and derivatives Avidin Assays for avidin Modified avidins Fluorescent avidin Radioactive avidin Avidin conjugates Immobilized avidin Antiavidin antibodies Preparation of biotin-binding proteins Avidin Streptavidin Egg-yolk protein Antibiotin antibodies Holocarboxylase synthetase Applications Affinity cytochemistry Affinity labeling Immunoassay Flow cytometry Affinity chromatography Blotting Volume [Chapter]" 18A[62], 18A[63], 18A[74], 57137], 62149], 62[50], 621511, 122[12], 1221131 18A[66], 18A[67], 18A[68], 18A[70], 18A[71], 109137], 18A[64], 18A[65], 34120], 46[72], 57[37], 62[54], 62155], 62163], 70[13], 83112], 92137], 109137], 138135], 151139] 62155], 62157], 83112], 92[20], 92[37], 121143], 121168], 133125], 149[11], 150138], 159156] 98122], 109137], 12415] 62155], 83112], 138135] 138135], 149111] 164125], 168154], 17014] 34120], 7118], 122113], 17014] 109137], 122[14], 122[15], 13517] 18A174], 18A[75], 18A[76], 57[37], 62151], 62[52], 62[53], 122112], 122113] 92[20], 109137], 133125] 85148], 92137], 122112], 150138] 62[53], 70113] 62155], 83[12], 92[37], 93120], 149111] 11216], 121168], 122[13], 12415], 125140] 18A[76], 122115] 18A[73], 34120], 122114] 1091371 62[56] 62157] 107[151 62155], 83112], 85[48], 103111], 1291191, 151139], 168154] 62[63], 164125] 73[33], 92120], 103127], 121143], 133125] 103[13], 150138] 34120], 109137], 122[14], 122[15], 12415], 125140], 13517], 17014] 138135], 159156] Vol.

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