Download e-book for kindle: Affinity Labeling: Affinity Labeling by Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

By Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

The seriously acclaimed laboratory typical, equipment in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. on the grounds that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this day - actually a necessary ebook for researchers in all fields of existence sciences.

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J. Milner-White and D. C. Watts, Biochem. J. 122, 727 (1971). k D. H. Buttlaire and M. Cohn, J. Biol. Chem. 249, 5733 (1974). K. A. Koetfler and G. P. Hess, Biochemistry 18, 5345 (1974). K. A. Koehler and G. E. Lienhard, Biochemistry 10, 2477 (1971). R. N. Lindquist and C. Terry, Arch. Biochem. Biophys. 160, 135 (1974). ° J. O. Westerik and R. Wolfenden, J. Biol. Chem. 247, 8195 (1971). P R. C. Thompson, Biochemistry 12, 47 (1973). J. O. Westerik a n d R . Wolfenden, J. Biol. Chem. 249, 6351 (1974).

238, 576 (1973). ee R. Wolfenden, Biochemistry 9, 3404 (1970). / / J . M. Chirgwin and E. A. Noltmann, Fed. Proc. 32, 667 (1973). gg D. Cassio, F. LeMoine, J. P. Waller, E. Sandrin, and R. A. Boissonas, Biochemistry 6, 827 (1967). hAW. B. Rowe, R. A. Ronzio, and A. Meister, Biochemistry 8, 2674 (1969). [2] TRANSITION STATE ANALOGS 25 active-site sulfhydryl group of this enzyme, thought to serve as a point of attachment for the developing carboxylic acid in the normal reaction catalyzed by this enzyme, is also a possible site of attachment of the inhibitor, as shown below.

5) If a second aliquot of fresh enzyme is added to a solution containing inactivated enzyme and excess inhibitor, the rate of inactivation of the second • G. Schoellman~ and E. Shaw, Biochem. Biophys. Res. Commun. 7, 36 (1962). [3] MECHANISM-BASED IRREVERSIBLE INHIBITORS 31 enzyme "pulse" should be the same as for the first. If its rate of inactivation is faster, it would be expected that an inhibitory metabolite is being formed in solution. (6) If stable, the product of the enzyme-inhibitor conversion should be an affinity labeling agent of the enzyme.

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